The TIGAR Polyclonal Antibody (CAB20416) is a valuable tool for researchers studying the TIGAR protein, a key regulator of cellular metabolism and stress response. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By targeting the TIGAR protein, this antibody allows for the precise detection and analysis of TIGAR expression in various cell types, making it ideal for investigations in cancer biology and metabolism research.TIGAR, or TP53 induced glycolysis and apoptosis regulator, is a crucial player in the cellular response to stress, modulating glucose metabolism and promoting cell survival under challenging conditions.
Its role in tumorigenesis and metabolic reprogramming has sparked interest in targeting TIGAR for cancer therapy and understanding its function in metabolic diseases. By studying the expression and function of TIGAR, researchers can gain insights into the mechanisms underlying these diseases and potentially identify novel therapeutic strategies.
Product Name:
TIGAR Rabbit Polyclonal Antibody
SKU:
CAB20416
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIGAR (NP_065108.1).
This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
Purification Method:
Affinity purification
Gene ID:
57103
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TIGAR antibody (CAB20416) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
