The TERT Polyclonal Antibody (CAB4774) is a vital tool for researchers studying TERT, a key enzyme involved in maintaining telomere length and cellular immortality. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in applications such as Western blotting.TERT, also known as telomerase reverse transcriptase, plays a crucial role in telomere maintenance and cell proliferation. Dysregulation of TERT has been implicated in various diseases, including cancer and aging-related disorders.
By targeting TERT with this antibody, researchers can investigate its expression and activity in different cell types, shedding light on its role in disease development and progression.The TERT Polyclonal Antibody is a valuable tool for researchers interested in exploring the molecular mechanisms underlying telomere biology and its implications for human health. Its high specificity, sensitivity, and reliability make it a preferred choice for studies in cancer research, aging, and other fields where TERT may be a significant factor.
Antibody Name:
Anti-TERT Antibody (CAB4774)
Antibody SKU:
CAB4774
Antibody Size:
50µL, 100µL
Application:
Western blotting
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A synthesized peptide derived from human TERT.
Application:
Western blotting
Recommended Dilution:
WB 1:500 - 1:2000
Reactivity:
Human, Mouse, Rat
Positive Samples:
Mouse testis, Mouse thymus, Rat liver, K-562
Immunogen:
A synthesized peptide derived from human TERT.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
