The Anti-TERT Antibody (CAB2979) is a valuable tool for researchers studying telomerase reverse transcriptase (TERT), an enzyme crucial for maintaining telomeres and controlling cell proliferation. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By targeting the TERT protein, this antibody enables accurate detection and analysis of TERT expression in various cell types, making it ideal for investigations in cancer biology, aging research, and regenerative medicine.TERT, a key player in telomere maintenance and cellular senescence, is known for its role in promoting immortalization and uncontrolled growth in cancer cells.
Its dysregulation is implicated in various cancers and age-related diseases, highlighting the importance of understanding TERT activity in disease development and potential therapeutic interventions. By studying TERT expression and function, researchers can further elucidate its impact on cellular processes and explore novel strategies for targeting TERT in cancer treatment and anti-aging therapies.
Product Name:
TERT Rabbit Polyclonal Antibody
SKU:
CAB2979
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human TERT (NP_937983.2).
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Purification Method:
Affinity purification
Gene ID:
7015
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HT-29 cells, using TERT antibody (CAB2979) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
