The Anti-TCF4/TCF7L2 Antibody (CAB20770) is a valuable tool for researchers studying the TCF4 and TCF7L2 proteins, which are transcription factors involved in regulating gene expression and cellular processes. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to TCF4 and TCF7L2 proteins, this antibody enables researchers to detect and analyze these transcription factors in various cell types, making it an essential reagent for studies in molecular biology and developmental biology.TCF4 and TCF7L2 are key players in the Wnt signaling pathway, which is critical for embryonic development, tissue homeostasis, and cancer progression.
Dysregulation of TCF4 and TCF7L2 has been linked to various diseases, including colorectal cancer, diabetes, and neurodevelopmental disorders. Research on the functions and regulation of these transcription factors is crucial for understanding their roles in these pathological conditions and identifying potential therapeutic targets.Overall, the Anti-TCF4/TCF7L2 Antibody (CAB20770) provides researchers with a reliable tool for investigating the functions and mechanisms of TCF4 and TCF7L2 proteins, advancing our knowledge of their roles in cellular processes and disease pathogenesis.
Product Name:
TCF4/TCF7L2 Polyclonal Antibody
SKU:
CAB20770
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 124-188 of human TCF4/TCF7L2 (NP_001354872.1).
Sequence:
TART LHFQ SGST HYSA YKTI EHQI AVQY LQMK WPLL DVQA GSLQ SRQA LKDA RSPS PAHI VSNK V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
TCF4; TCF-4; TCF4/TCF7L2
Positive Sample:
SW620,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
nucleoplasm, nucleus, PML body
Calculated MW:
68kDa
Observed MW:
60kDa/75kDa
This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6934
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody (CAB20770) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
