The Syntaxin-4 Polyclonal Antibody (CAB5996) is a valuable tool for researchers studying Syntaxin-4, a protein involved in intracellular membrane fusion and trafficking processes. This antibody, generated in rabbits, exhibits high specificity for Syntaxin-4 in human samples and is validated for use in Western blot applications. By binding to Syntaxin-4, this antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in cell biology, neuroscience, and endocrinology.
Syntaxin-4 is essential for vesicle fusion events in various cellular processes, including neurotransmitter release, hormone secretion, and cell migration. Dysfunction of Syntaxin-4 has been implicated in diseases such as diabetes, neurodegenerative disorders, and immune disorders. Therefore, further investigation into the role of Syntaxin-4 in these conditions is crucial for developing targeted therapies and understanding disease pathology at the molecular level.
Product Name:
Syntaxin 4 Rabbit Monoclonal Antibody
SKU:
CAB5996
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Syntaxin 4 (NP_004595.2)).
basolateral plasma membrane, cytosol, dendritic spine, endosome, extracellular exosome, extracellular space, glutamatergic synapse, lateral loop, myelin sheath adaxonal region, neuron projection membrane, perinuclear region of cytoplasm, plasma membrane, postsynapse, presynaptic active zone membrane, SNARE complex, synapse, synaptic vesicle, trans-Golgi network
Calculated MW:
34kDa
Observed MW:
34kDa
Enables sphingomyelin phosphodiesterase activator activity. Involved in several processes, including cornified envelope assembly; positive regulation of immune effector process; and positive regulation of protein localization. Located in several cellular components, including basolateral plasma membrane; cytoplasmic vesicle; and lamellipodium. Part of SNARE complex. Is active in glutamatergic synapse and postsynapse.
Purification Method:
Affinity purification
Gene ID:
6810
Clone Number:
ARC2113
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Syntaxin 4 Rabbit mAb (CAB5996) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
