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SUPT16H (SPT16) Rabbit Polyclonal Antibody (CAB18696)

SKU:
CAB18696
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CDC68
SPT16
FACTP140
SPT16/CDC68
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SUPT16H (SPT16) Rabbit Polyclonal Antibody
SKU:CAB18696
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 520-695 of human SUPT16H (SPT16) (NP_009123.1).
Sequence:KEPH IREM KIYI DKKY ETVI MPVF GIAT PFHI ATIK NISM SVEG DYTY LRIN FYCP GSAL GRNE GNIF PNPE ATFV KEIT YRAS NIKA PGEQ TVPA LNLQ NAFR IIKE VQKR YKTR EAEE KEKE GIVK QDSL VINL NRSN PKLK DLYI RPNI AQKR MQGS LEAH VNGF RFTS VRGD
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:CDC68; SPT16; NEDDFAC; FACTP140; SPT16/CDC68; SUPT16H (SPT16)
Positive Sample:HCT 116,C6
Cellular Localization:nucleoplasm, nucleus
Calculated MW:120kDa
Observed MW:140kDa

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.

Purification Method:Affinity purification
Gene ID:11198
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using SUPT16H (SPT16) Rabbit pAb (CAB18696) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 180s.

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