The SPT16 Polyclonal Antibody (CAB18696) is a valuable tool for researchers studying SPT16H, a key component of the FACT (Facilitates Chromatin Transcription) complex involved in transcription elongation and chromatin remodeling. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the SPT16H protein, this antibody allows for the detection and analysis of SPT16H in various cell types, making it an essential tool for studies in molecular biology and gene regulation.SPT16H is essential for the proper functioning of the FACT complex, which is responsible for maintaining chromatin structure during transcription.
Dysregulation of SPT16H has been linked to various diseases, including cancer and developmental disorders. Understanding the role of SPT16H in gene expression and chromatin remodeling is critical for unraveling the mechanisms underlying these diseases and identifying potential targets for therapeutic intervention. The SPT16 Polyclonal Antibody provides researchers with a precise and reliable tool for investigating the function of SPT16H in cellular processes, making it an invaluable resource in the field of molecular biology.
Product Name:
SUPT16H (SPT16) Rabbit Polyclonal Antibody
SKU:
CAB18696
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 520-695 of human SUPT16H (SPT16) (NP_009123.1).
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.
Purification Method:
Affinity purification
Gene ID:
11198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SUPT16H (SPT16) Rabbit pAb (CAB18696) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 180s.
