The Anti-SUN2 Antibody (CAB19782) is a highly specific polyclonal antibody designed for research involving SUN2, a protein involved in nuclear membrane architecture and function. The antibody is raised in rabbits and validated for use in various applications, including Western blot and immunofluorescence.SUN2 is a key component of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which plays important roles in nuclear migration, chromatin organization, and mechanotransduction. The Anti-SUN2 Antibody binds specifically to SUN2, allowing for the visualization and analysis of its expression and localization in different cell types.
Research on SUN2 is crucial for understanding its functions in nuclear dynamics and cell biology. Dysregulation of SUN2 has been implicated in various diseases, including muscular dystrophy and cancer. Therefore, studying SUN2 using the Anti-SUN2 Antibody may provide valuable insights into the molecular mechanisms underlying these conditions and aid in the development of potential therapeutic strategies.
Product Name:
SUN2 Rabbit Monoclonal Antibody
SKU:
CAB19782
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 618-717 of human SUN2 (Q9UH99).
SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).
Purification Method:
Affinity purification
Gene ID:
25777
Clone Number:
ARC2311
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SUN2 antibody (CAB19782) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
