The Steroidogenic Factor 1 (SF-1/NR5A1) Polyclonal Antibody (CAB19277) is an essential tool for researchers investigating SF-1/NR5A1, a transcription factor involved in the regulation of steroidogenesis and reproductive development. This antibody, produced in rabbits, exhibits a high degree of reactivity with human samples and has been rigorously validated for use in Western blotting applications. By specifically targeting the SF-1/NR5A1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an indispensable resource for studies in endocrinology, reproductive biology, and developmental biology.
SF-1/NR5A1 plays a pivotal role in the control of steroid hormone production, as well as the development and function of reproductive tissues. Dysregulation of SF-1/NR5A1 has been implicated in various endocrine disorders, including disorders of sexual development, adrenal insufficiency, and reproductive abnormalities. By elucidating the molecular mechanisms underlying SF-1/NR5A1 function, researchers can gain valuable insights into these conditions and pave the way for the development of targeted therapeutic interventions.
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.
Purification Method:
Affinity purification
Gene ID:
2516
Clone Number:
ARC2457
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Steroidogenic factor-1 (SF-1/NR5A1) antibody (CAB19277) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.