The SPI1 Polyclonal Antibody (CAB20461) is a valuable tool for researchers studying SPI1, a transcription factor involved in regulating hematopoiesis and immune cell development. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting SPI1 protein levels in human samples. Validated for use in Western blot applications, it enables researchers to analyze SPI1 expression in various cell types, making it an essential reagent for studies in immunology and oncology.SPI1, also known as PU.1, plays a crucial role in the differentiation and function of immune cells, such as macrophages, B cells, and dendritic cells. Dysregulation of SPI1 expression has been implicated in various diseases, including leukemia, autoimmune disorders, and inflammatory conditions.
Investigating the function of SPI1 with this antibody can provide insights into the mechanisms underlying these diseases and potentially lead to the development of novel therapeutic strategies targeting SPI1 signaling pathways.Overall, the SPI1 Polyclonal Antibody (CAB20461) is a reliable tool for researchers seeking to explore the role of SPI1 in immune cell development and disease pathogenesis. Its high specificity and sensitivity make it a valuable asset in advancing our understanding of immune regulation and its implications for human health.
Product Name:
SPI1 Rabbit Polyclonal Antibody
SKU:
CAB20461
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 17-98 of human SPI1 (NP_003111.2).
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6688
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SPI1 Rabbit pAb (CAB20461) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC):JurkatExposure time: 30s.
