The SMURF2 Monoclonal Antibody (CAB2278) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the SMURF2 protein, this antibody enables the detection and analysis of SMURF2 expression levels in various cell types.SMURF2 is known for its role in regulating diverse cellular functions, including cell proliferation, differentiation, and apoptosis. Dysfunction of SMURF2 has been implicated in various diseases, making it a potential target for therapeutic interventions.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SMURF2 Monoclonal Antibody
SKU:
CAB2278
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1897
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables SMAD binding activity; identical protein binding activity; and ubiquitin-protein transferase activity. Involved in negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent SMAD protein catabolic process. Located in nuclear speck. Part of ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
64750
RRID
AB_2862986
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMURF2 Rabbit mAb (CAB2278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon using SMURF2 Rabbit mAb (CAB2278) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
