The SMC4 Monoclonal Antibody (CAB3559) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot and immunofluorescence applications.SMC4, also known as structural maintenance of chromosomes 4, plays a crucial role in maintaining genome stability and proper chromosome organization. Dysregulation of SMC4 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMC4 Monoclonal Antibody
SKU:
CAB3559
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2042
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAPC, CAP-C, SMC-4, SMC4L1, SMC4
Positive Sample:
HT-29, NIH/3T3, Raji, C6, Mouse testis, Mouse thymus, Rat testis
Cellular Localization:
Chromosome, Cytoplasm, Nucleus.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.
Purification Method
Affinity purification
Gene ID
10051
RRID
AB_2863086
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMC4 Rabbit mAb (CAB3559) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb (CAB3559) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (CAB3559) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
