The SLC12A1 (NKCC2) Polyclonal Antibody (CAB20933) is a valuable tool for researchers studying the SLC12A1 protein, also known as sodium-potassium-chloride cotransporter 2 (NKCC2). This antibody, produced by Assay Genie, is raised in rabbits and has been validated for use in Western blot applications. SLC12A1 is a key regulator of ion transport in the kidney, specifically in the thick ascending limb of the loop of Henle. Dysfunction in SLC12A1 has been implicated in various kidney disorders, making it a target of interest for researchers in nephrology and kidney disease. By specifically binding to SLC12A1, this antibody allows for the detection and analysis of the protein in different cell types and tissues.
Its high reactivity with human samples makes it a versatile tool for studies in renal physiology and pathology. Additionally, understanding the function and regulation of SLC12A1 is essential for the development of potential therapies targeting kidney diseases. Overall, the SLC12A1 (NKCC2) Polyclonal Antibody (CAB20933) from Assay Genie is a valuable resource for researchers investigating the role of SLC12A1 in kidney function and disease, providing insights that may lead to new treatment strategies in the future.
Product Name:
SLC12A1/NKCC2 Monoclonal Antibody
SKU:
CAB20933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 601-800 of human SLC12A1 / NKCC2 (Q13621).
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.
Purification Method:
Affinity purification
Gene ID:
6557
Clone Number:
ARC2915
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SLC12A1/NKCC2 antibody (CAB20933) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
