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SF3B1 Rabbit Monoclonal Antibody (CAB9737)

SKU:
CAB9737
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
Hsh155
MDS
PRP10
PRPF10
SAP155
SF3b155
Research Area:
Epigenetics and Nuclear Signaling
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SF3B1 Rabbit Monoclonal Antibody
SKU:CAB9737
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).
Sequence:MAKI AKTH EDIE AQIR EIQG KKAA LDEA QGVG LDST GYYD QEIY GGSD SRFA GYVT SIAA TELE DDDD DYSS STSL LGQK KPGY HAPV ALLN DIPQ STEQ
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155; SF3B1
Positive Sample:HeLa,NIH/3T3,Jurkat,Mouse brain
Conjugate:Unconjugated
Cellular Localization:B-WICH complex, Nuclear speck, Nucleolus, Nucleoplasm, Nucleus
Calculated MW:146kDa

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:23451
Clone Number:ARC1724
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using SF3B1 Rabbit mAb (CAB9737) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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