The Anti-SF3B1 Antibody (CAB9737) is a valuable tool for researchers studying SF3B1, a critical protein involved in RNA splicing. This antibody, produced in rabbits, is highly specific to human SF3B1 and is validated for use in Western blot applications. By binding to the SF3B1 protein, this antibody allows for the detection and analysis of SF3B1 in a variety of cell types, making it ideal for research in molecular biology and cancer biology.
SF3B1 is a key component of the spliceosome complex, playing a crucial role in the splicing of pre-mRNA molecules. Dysregulation of SF3B1 has been implicated in various diseases, including cancer. By studying SF3B1 using this antibody, researchers can gain valuable insights into the mechanisms underlying disease pathology and potentially identify new therapeutic targets for the treatment of cancer and other disorders.
Product Name:
SF3B1 Rabbit Monoclonal Antibody
SKU:
CAB9737
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
23451
Clone Number:
ARC1724
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using SF3B1 Rabbit mAb (CAB9737) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
