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SELENOK Rabbit Polyclonal Antibody (CAB20560)

SKU:
CAB20560
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
SELK
HSPC030
HSPC297
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SELENOK Rabbit Polyclonal Antibody
SKU:CAB20560
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-94 of human SELENOK (NP_067060.2).
Sequence:MVYI SNGQ VLDS RSQS PWRL SLIT DFFW GIAE FVVL FFKT LLQQ DVKK RRSY GNSS DSRY DDGR GPPG NPPR RMGR INHL RGPS PPPM AGGU GR
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:SELK; HSPC030; HSPC297; SELENOK
Positive Sample:Mouse spleen,Mouse heart
Conjugate:Unconjugated
Cellular Localization:endoplasmic reticulum, Golgi apparatus, plasma membrane
Calculated MW:11kDa
Observed MW:11kDa

The protein encoded by this gene belongs to the selenoprotein K family. It is a transmembrane protein that is localized in the endoplasmic reticulum (ER), and is involved in ER-associated degradation (ERAD) of misfolded, glycosylated proteins. It also has a role in the protection of cells from ER stress-induced apoptosis. Knockout studies in mice show the importance of this gene in promoting Ca(2+) flux in immune cells and mounting effective immune response. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Pseudogenes of this locus have been identified on chromosomes 6 and 19.

Purification Method:Affinity purification
Gene ID:58515
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using SELENOK antibody (CAB20560) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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