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SARS-CoV-2 Spike S2 ECD Rabbit Polyclonal Antibody (CAB20138)

SKU:
CAB20138
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SARS-CoV-2 Spike S2 ECD Rabbit Polyclonal Antibody
SKU:CAB20138
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,SARS-CoV-2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 686-1214 of coronavirus Spike S2 ECD (YP_009724390.1).
Sequence:SVAS QSII AYTM SLGA ENSV AYSN NSIA IPTN FTIS VTTE ILPV SMTK TSVD CTMY ICGD STEC SNLL LQYG SFCT QLNR ALTG IAVE QDKN TQEV FAQV KQIY KTPP IKDF GGFN FSQI LPDP SKPS KRSF IEDL LFNK VTLA DAGF IKQY GDCL GDIA ARDL ICAQ KFNG LTVL PPLL TDEM IAQY TSAL LAGT ITSG WTFG AGAA LQIP FAMQ MAYR FNGI GVTQ NVLY ENQK LIAN QFNS AIGK IQDS LSST ASAL GKLQ DVVN QNAQ ALNT LVKQ LSSN FGAI SSVL NDIL SRLD KVEA EVQI DRLI TGRL QSLQ TYVT QQLI RAAE IRAS ANLA ATKM SECV LGQS KRVD FCGK GYHL MSFP QSAP HGVV FLHV TYVP AQEK NFTT APAI CHDG KAHF PREG VFVS NGTH WFVT QRNF YEPQ IITT DNTF VSGN CDVV IGIV NNTV YDPL QPEL DSFK EELD KYFK NHTS PDVD LGDI SGIN ASVV NIQK EIDR LNEV AKNL NESL IDLQ ELGK YEQY IKWP W
Tested Applications:WB IF/ICC IP ELISA DB
Recommended Dilution:DB,1:500 - 1:2000 WB,1:2000 - 1:6000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:spike glycoprotein; SARS-CoV-2 Spike S2 ECD
Positive Sample:293T
Calculated MW:141kDa
Observed MW:75kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Purification Method:Affinity purification
Gene ID:43740568
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD Rabbit pAb (CAB20138) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.

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