The Anti-SARS-CoV-2 Spike S2 ECD Antibody (CAB20138) is a crucial tool for research on the SARS-CoV-2 virus, specifically targeting the spike protein S2 extracellular domain (ECD). This antibody, produced and validated for use in various research applications including ELISA and Western blot, is highly specific and sensitive in detecting the SARS-CoV-2 spike protein.The SARS-CoV-2 spike protein is a key target for vaccine development and therapeutic interventions against COVID-19. By targeting the S2 ECD region of the spike protein, this antibody provides researchers with a valuable tool for studying the structure and function of the virus, as well as potential interactions with host cells.
Understanding the immune response to SARS-CoV-2 and developing effective strategies to combat the virus are essential in the fight against the COVID-19 pandemic. The Anti-SARS-CoV-2 Spike S2 ECD Antibody (CAB20138) plays a critical role in advancing research in this area, offering a reliable and specific tool for studying the SARS-CoV-2 spike protein and its implications for vaccine and therapeutic development.
DB,1:500 - 1:2000 WB,1:2000 - 1:6000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
spike glycoprotein; SARS-CoV-2 Spike S2 ECD
Positive Sample:
293T
Calculated MW:
141kDa
Observed MW:
75kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method:
Affinity purification
Gene ID:
43740568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD Rabbit pAb (CAB20138) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.
