The Anti-SARS-CoV-2 Spike S2 Antibody (CAB20284) is a highly specific and sensitive antibody designed for research involving the SARS-CoV-2 virus, specifically targeting the spike S2 protein. This polyclonal antibody, raised in rabbits, has been validated for use in various applications, including immunohistochemistry, ELISA, and Western blot.The SARS-CoV-2 spike S2 protein plays a crucial role in viral entry into host cells, making it a key target for research and vaccine development. By using this antibody, researchers can detect and analyze the presence of the spike S2 protein in samples, allowing for a better understanding of viral infection and potential therapeutic strategies.
With its high reactivity and specificity for the SARS-CoV-2 spike S2 protein, this antibody is essential for studies in virology, infectious diseases, and vaccine development. Its proven performance in various applications makes it a valuable tool for researchers working towards combating the COVID-19 pandemic.
Product Name:
SARS-CoV-2 Spike S2 Rabbit Polyclonal Antibody
SKU:
CAB20284
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
SARS-CoV-2
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of SARS-CoV-2 Spike S2 (YP_009724390.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
spike glycoprotein; SARS-CoV-2 Spike S2
Positive Sample:
293T
Calculated MW:
141kDa
Observed MW:
36kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method:
Affinity purification
Gene ID:
43740568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike S2 Protein, using SARS-CoV-2 Spike S2 Rabbit pAb (CAB20284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
