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SARS-CoV-2 Spike S1 Rabbit Polyclonal Antibody (CAB20136)

SKU:
CAB20136
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SARS-CoV-2 Spike S1 Rabbit Polyclonal Antibody
SKU:CAB20136
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 11-682 of coronavirus Spike S1 (YP_009724390.1).
Sequence:VSSQ CVNL TTRT QLPP AYTN SFTR GVYY PDKV FRSS VLHS TQDL FLPF FSNV TWFH AIHV SGTN GTKR FDNP VLPF NDGV YFAS TEKS NIIR GWIF GTTL DSKT QSLL IVNN ATNV VIKV CEFQ FCND PFLG VYYH KNNK SWME SEFR VYSS ANNC TFEY VSQP FLMD LEGK QGNF KNLR EFVF KNID GYFK IYSK HTPI NLVR DLPQ GFSA LEPL VDLP IGIN ITRF QTLL ALHR SYLT PGDS SSGW TAGA AAYY VGYL QPRT FLLK YNEN GTIT DAVD CALD PLSE TKCT LKSF TVEK GIYQ TSNF RVQP TESI VRFP NITN LCPF GEVF NATR FASV YAWN RKRI SNCV ADYS VLYN SASF STFK CYGV SPTK LNDL CFTN VYAD SFVI RGDE VRQI APGQ TGKI ADYN YKLP DDFT GCVI AWNS NNLD SKVG GNYN YLYR LFRK SNLK PFER DIST EIYQ AGST PCNG VEGF NCYF PLQS YGFQ PTNG VGYQ PYRV VVLS FELL HAPA TVCG PKKS TNLV KNKC VNFN FNGL TGTG VLTE SNKK FLPF QQFG RDIA DTTD AVRD PQTL EILD ITPC SFGG VSVI TPGT NTSN QVAV LYQD VNCT EVPV AIHA DQLT PTWR VYST GSNV FQTR AGCL IGAE HVNN SYEC DIPI GAGI CASY QTQT NSPR
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:ELISA,1:20000-1:80000 WB,1:500 - 1:1000 IF/ICC,1:100 - 1:500 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:spike glycoprotein; SARS-CoV-2 Spike S1
Positive Sample:293T
Calculated MW:141kDa
Observed MW:110kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Purification Method:Affinity purification
Gene ID:43740568
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using SARS-CoV-2 Spike S1 Rabbit pAb (CAB20136) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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