The Anti-SARS-CoV-2 Spike RBD Antibody (CAB20135) is a highly specific and sensitive antibody designed for research involving the Spike Protein Receptor Binding Domain (RBD) of the SARS-CoV-2 virus. This polyclonal antibody, raised in rabbits, is validated for use in various applications such as ELISA, Western blot, and immunohistochemistry.The SARS-CoV-2 Spike RBD is a key component of the virus responsible for binding to host cells and facilitating viral entry. Understanding the interaction between the Spike RBD and host cell receptors is crucial for developing targeted therapies, vaccines, and diagnostic tools for COVID-19.
This antibody enables detection and analysis of the Spike RBD protein in samples from infected individuals, making it an invaluable tool for research in virology, immunology, and infectious diseases.By studying the Spike RBD protein and its interactions with host cells, researchers can gain insights into the mechanisms of viral infection and transmission, as well as develop strategies to combat the spread of COVID-19. The Anti-SARS-CoV-2 Spike RBD Antibody (CAB20135) is a valuable resource for advancing our understanding of the SARS-CoV-2 virus and developing effective countermeasures against the ongoing global pandemic.
Product Name:
SARS-CoV-2 Spike RBD Rabbit Polyclonal Antibody
SKU:
CAB20135
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
SARS-CoV-2
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 332-550 of coronavirus Spike RBD (YP_009724390.1).
ELISA,1:50000-1:200000 WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
spike glycoprotein; SARS-CoV-2 Spike RBD
Positive Sample:
293T
Calculated MW:
141kDa
Observed MW:
30kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method:
Affinity purification
Gene ID:
43740568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein,using SARS-CoV-2 Spike RBD Rabbit pAb (CAB20135) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
