The Anti-SARS-CoV-2 Spike ECD Antibody (CAB20497) is a crucial tool for research involving the Spike protein of the SARS-CoV-2 virus, responsible for the COVID-19 pandemic. This polyclonal antibody, produced against the extracellular domain of the Spike protein, is highly specific and exhibits strong reactivity with human samples.Validated for use in various applications such as Western blot and immunohistochemistry, this antibody allows for the detection and analysis of the SARS-CoV-2 Spike protein in different cell types and tissues. Its high affinity for the Spike protein makes it an ideal choice for studies focusing on viral entry mechanisms, vaccine development, and antiviral drug screening.
The Spike protein of SARS-CoV-2 plays a crucial role in viral attachment and entry into host cells, making it a prime target for research aiming to understand virus-host interactions and develop effective therapeutics. By using this antibody, researchers can further their understanding of the SARS-CoV-2 virus and contribute to the development of strategies to combat the ongoing pandemic.
Product Name:
SARS-CoV-2 Spike ECD Rabbit Polyclonal Antibody
SKU:
CAB20497
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
SARS-CoV-2
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 11-1208 of coronavirus Spike ECD (YP_009724390.1).
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method:
Affinity purification
Gene ID:
43740568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using SARS-CoV-2 Spike ECD Rabbit pAb (CAB20497) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
