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SARS-CoV-2 Spike Rabbit Polyclonal Antibody (CAB20137)

SKU:
CAB20137
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SARS-CoV-2 Spike Rabbit Polyclonal Antibody
SKU:CAB20137
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of coronavirus Spike (YP_009724390.1).
Sequence:PGTN TSNQ VAVL YQDV NCTE VPVA IHAD QLTP TWRV YSTG SNVF QTRA GCLI GAEH VNNS YECD IPIG AGIC ASYQ TQTN SPRR ARSV ASQS IIAY TMSL G
Tested Applications:WB IF/ICC IP ELISA DB
Recommended Dilution:DB,1:500 - 1:2000 WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:spike glycoprotein; SARS-CoV-2 Spike
Positive Sample:293T
Calculated MW:141kDa
Observed MW:110kDa/180kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Purification Method:Affinity purification
Gene ID:43740568
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T and transfected 293T-S1（His-tag), using SARS-CoV-2 Spike Rabbit pAb (CAB20137) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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