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SARS-CoV-2 ORF9b Rabbit Polyclonal Antibody (CAB20260)

SKU:
CAB20260
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

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SARS-CoV-2 ORF9b Rabbit Polyclonal Antibody (CAB20260)

The Anti-SARS-CoV-2 ORF9b Antibody (CAB20260) is a crucial tool for researchers studying the novel coronavirus, specifically targeting the ORF9b protein implicated in viral pathogenesis. This antibody, generated in rabbits, exhibits high reactivity with samples from SARS-CoV-2-infected individuals and has been validated for use in various applications, including Western blotting and immunofluorescence.ORF9b is a non-structural protein encoded by the SARS-CoV-2 genome, playing a vital role in viral replication and immune evasion mechanisms. Understanding the function of ORF9b is essential for developing effective antiviral strategies and vaccines against COVID-19.

The Anti-SARS-CoV-2 ORF9b Antibody enables researchers to detect and analyze the presence of this protein in infected cells, facilitating insights into viral pathogenesis and potential therapeutic targets.In the current global health crisis, research on SARS-CoV-2 is paramount, and the Anti-SARS-CoV-2 ORF9b Antibody serves as a valuable tool for advancing our understanding of this virus and developing strategies to combat its spread. By targeting the ORF9b protein, researchers can uncover new insights into the mechanisms underlying COVID-19 and contribute to the ongoing efforts to control the pandemic.

Product Name:SARS-CoV-2 ORF9b Rabbit Polyclonal Antibody
SKU:CAB20260
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of coronavirus ORF9b (P0DTD2).
Sequence:MDPK ISEM HPAL RLVD PQIQ LAVT RMEN AVGR DQNN VGPK VYPI ILRL GSPL SLNM ARKT LNSL EDKA FQLT PIAV QMTK LATT EELP DEFV VVTV K
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Positive Sample:293T
Calculated MW:11kDa
Observed MW:12-14kDa

SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the causative agent of the COVID19 pandemic.?? SARS-CoV-2 and SARS-CoVshare many proteins common in other CoVs, including 4 majorstructural proteins (S, E, M, and N proteins) and 16 nonstructuralproteins (nsp1-16), they possess a unique set of proteins, namelyorf3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b。The SARS-CoV-2 genome encodes for a small accessory protein termed Orf9b,?? which targets the mitochondrial outer membrane protein TOM70 in infected cells.This 98-amino acid (aa) protein is encoded by analternative open reading frame (ORF) within the N gene and istranslated via a leaky scanning mechanism during translationCrystal structures of orf9b alone revealed a homodimericβ-strand-rich structure? with a hydro_x005fphobic central tunnel for lipid binding, consistent with the role oforf9b in the mature virion assembly

Purification Method:Affinity purification
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells 293T transfected with ORF9b Protein, using SARS-CoV-2 ORF9b Rabbit pAb (CAB20260) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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