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SARS-CoV-2 ORF3A Rabbit Polyclonal Antibody (CAB20234)

SKU:
CAB20234
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

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Product Name:SARS-CoV-2 ORF3A Rabbit Polyclonal Antibody
SKU:CAB20234
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus ORF3A (YP_009724391.1).
Sequence:GLEA PFLY LYAL VYFL QSIN FVRI IMRL WLCW KCRS KNPL LYDA NYFL CWHT NCYD YCIP YNSV TSSI VITS GDGT TSPI SEHD YQIG GYTE KWES GVKD C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:6000
Positive Sample:293T
Calculated MW:31kDa
Observed MW:35kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF3a encodes a viral accessory protein. Based on its similarity to other coronavirus proteins, ORF3a protein is thought to be a protein with ion channel activity (viroporin) that activates the NLRP3 inflammasome. ORF3a may also play a role in virus replication and pathogenesis.

Purification Method:Affinity purification
Gene ID:43740569
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using SARS-CoV-2 ORF3A Rabbit pAb (CAB20234) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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