The Anti-SARS-CoV-2 Nucleoprotein Antibody (CAB18797) is a crucial tool for research related to the SARS-CoV-2 virus, responsible for the COVID-19 pandemic. This antibody, produced and validated for use in various applications, including ELISA and Western blot, specifically targets the nucleoprotein of the virus. The SARS-CoV-2 nucleoprotein is essential for viral replication and plays a key role in viral assembly, making it a prime target for diagnostic and therapeutic development.
By detecting and analyzing the nucleoprotein, researchers can better understand the viral lifecycle and develop strategies to combat its spread. With its high reactivity with human samples, this antibody is a valuable tool for studies in virology, infectious diseases, and vaccine development. Its specificity and sensitivity make it an essential component in the fight against the COVID-19 pandemic and furthering our understanding of this novel coronavirus.
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 DB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Positive Sample:
293T transfected with Nucleoprotein,Recombinant SARS-CoV-2 Nucleoprotein
Observed MW:
50kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.
Purification Method:
Affinity purification
Gene ID:
43740575
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein,using SARS-CoV-2 Nucleoprotein Rabbit pAb (CAB18797) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
