Description
SARS-CoV-2 NSP8 Antibody (CAB20202)
The SARS-CoV-2 NSP8 Antibody (CAB20202) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed for research purposes, particularly in the field of virology and infectious diseases. The antibody is highly reactive with human samples and has been validated for various applications, including Western blotting. By binding to the NSP8 protein, this antibody enables accurate detection and analysis of this key viral component in different cell types. Its specificity and reliability make it an essential tool for researchers studying the pathogenesis and potential treatments for COVID-19.The NSP8 protein is known to play a vital role in viral replication and the overall life cycle of the SARS-CoV-2 virus.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP8 Antibody |
| SKU: | CAB20202 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | AIAS EFSS LPSY AAFA TAQE AYEQ AVAN GDSE VVLK KLKK SLNV AKSE FDRD AAMQ RKLE KMAD QAMT QMYK QARS EDKR AKVT SAMQ TMLF TMLR KLDN DALN NIIN NARD GCVP LNII PLTT AAKL MVVI PDYN TYKN TCDG TTFT YASA LWEI QQVV DADS KIVQ LSEI SMDN SPNL AWPL IVTA LRAN SAVK LQ | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 141kDa |
| Observed MW: | 25kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP8 Protein, using SARS-CoV-2 NSP8 Rabbit pAb (CAB20202) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
