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SARS-CoV-2 NSP4 Antibody (CAB20281)

SKU:
CAB20281
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

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SARS-CoV-2 NSP4 Rabbit Polyclonal Antibody (CAB20281)

The Anti-SARS-CoV-2 NSP4 Antibody (CAB20281) is a valuable tool for research into the novel coronavirus SARS-CoV-2. This polyclonal antibody, raised in rabbits, is highly specific and reactive with samples from individuals infected with SARS-CoV-2. Validated for use in various applications including ELISA, Western blot, and immunofluorescence, this antibody binds to NSP4, a non-structural protein essential for viral replication.NSP4 plays a crucial role in the life cycle of SARS-CoV-2, making it a promising target for antiviral drug development and vaccine research.

By studying the interaction of NSP4 with host cells, researchers can gain valuable insights into the mechanisms of viral infection and potentially identify new therapeutic strategies to combat COVID-19. This antibody is an indispensable tool for scientists working in virology, immunology, and infectious diseases, contributing to our understanding of SARS-CoV-2 pathogenesis and the development of effective countermeasures.

Product Name:SARS-CoV-2 NSP4 Rabbit Polyclonal Antibody
SKU:CAB20281
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of coronavirus NSP4 (YP_009725300.1).
Sequence:RRVV FNGV SFST FEEA ALCT FLLN KEMY LKLR SDVL LPLT QYNR YLAL YNKY KYFS GAMD TTSY REAA CCHL AKAL NDFS NSGS DVLY QPPQ TSIT SAVL Q
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Positive Sample:293T,
Calculated MW:794kDa
Observed MW:28kDa/11kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 NSP4 using SARS-CoV-2 NSP4 Rabbit pAb(CAB20281) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 20 μg/10 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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