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SARS-CoV-2 NSP16 Rabbit Polyclonal Antibody (CAB20283)

SKU:
CAB20283
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

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SARS-CoV-2 NSP16 Rabbit Polyclonal Antibody (CAB20283)

The Anti-SARS-CoV-2 NSP16 Antibody (CAB20283) is a valuable tool for researchers studying the novel coronavirus, SARS-CoV-2. This polyclonal antibody, raised in rabbits, is highly specific for the NSP16 protein of the virus, a crucial component for viral replication. Validated for use in various applications, including Western blot and immunofluorescence, this antibody enables accurate detection and analysis of SARS-CoV-2 NSP16 in cell samples.NSP16 is a methyltransferase enzyme that plays a key role in viral RNA modification and evasion of the host immune response.

Targeting NSP16 with this antibody allows for a better understanding of the mechanisms underlying viral replication and pathogenicity of SARS-CoV-2. This antibody is essential for research into antiviral drug development, vaccine design, and understanding the immune response to COVID-19.By utilizing the Anti-SARS-CoV-2 NSP16 Antibody (CAB20283), researchers can gain valuable insights into the molecular mechanisms of SARS-CoV-2 infection, helping to advance our knowledge and response to this global health crisis.

Product Name:SARS-CoV-2 NSP16 Rabbit Polyclonal Antibody
SKU:CAB20283
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 236-290 of coronavirus NSP16 (YP_009725302.1).
Sequence:RLTL GVYD YLVS TQEF RYMN SQGL LPPK NSID AFKL NIKL LGVG GKPC IKVA TVQ
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:6000
Positive Sample:293T
Calculated MW:794kDa
Observed MW:37kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells and 293T transfected with NSP16 Protein, using SARS-CoV-2 NSP16 Rabbit pAb (CAB20283) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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