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SARS-CoV-2 NSP15 Rabbit Polyclonal Antibody (CAB20282)

SKU:
CAB20282
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

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SARS-CoV-2 NSP15 Rabbit Polyclonal Antibody (CAB20282)

The Anti-SARS-CoV-2 NSP15 Antibody (CAB20282) is a valuable tool for research involving the NSP15 protein of the SARS-CoV-2 virus, which is essential for viral replication and virulence. This antibody, produced in rabbits, is highly specific and reacts with samples from SARS-CoV-2 infected individuals, making it a reliable tool for studying the virus in various research applications.NSP15 is a key enzyme in the coronavirus life cycle, playing a crucial role in viral RNA processing and evasion of the host immune response. By targeting NSP15, researchers can gain insights into the mechanisms of SARS-CoV-2 infection and potentially identify targets for antiviral therapies.

This antibody is validated for use in techniques such as Western blot and immunofluorescence, providing researchers with a versatile tool for studying the SARS-CoV-2 virus and its interactions with host cells.Understanding the function of NSP15 is essential for developing effective strategies to combat the COVID-19 pandemic. This antibody is a valuable resource for researchers working towards this goal, providing a reliable means of studying the SARS-CoV-2 virus and its proteins in the pursuit of effective treatments and prevention measures.

Product Name:SARS-CoV-2 NSP15 Rabbit Polyclonal Antibody
SKU:CAB20282
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 6600-6700 of coronavirus NSP15 (YP_009724389.1).
Sequence:VKGL QPSV GPKQ ASLN GVTL IGEA VKTQ FNYY KKVD GVVQ QLPE TYFT QSRN LQEF KPRS QMEI DFLE LAMD EFIE RYKL EGYA FEHI VYGD FSHS QLGG L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:6000
Positive Sample:293T
Calculated MW:794kDa
Observed MW:42kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells and 293T transfected with NSP15 Protein, using SARS-CoV-2 NSP15 Rabbit pAb (CAB20282) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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