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SARS-CoV-2 NSP1 Rabbit Polyclonal Antibody (CAB20200)

SKU:
CAB20200
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

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SARS-CoV-2 NSP1 Rabbit Polyclonal Antibody (CAB20200)

The Anti-SARS-CoV-2 NSP1 Antibody (CAB20200) is a valuable tool for researchers studying the novel coronavirus SARS-CoV-2. This polyclonal antibody, raised in rabbits, is highly reactive with samples from individuals infected with SARS-CoV-2 and is validated for use in various applications, including Western blotting.The NSP1 protein of SARS-CoV-2 is a non-structural protein involved in viral replication and immune evasion. By targeting this specific protein, researchers can gain insights into how the virus manipulates host cells and evades the immune response.

This antibody enables the detection and analysis of NSP1 in different cell types, making it a versatile tool for studies in virology and immunology.Understanding the role of NSP1 in the pathogenesis of SARS-CoV-2 is crucial for developing effective treatments and vaccines against COVID-19. By studying the interactions between NSP1 and host cell proteins, researchers can identify potential targets for antiviral therapies and contribute to the global effort to combat the ongoing pandemic.

Product Name:SARS-CoV-2 NSP1 Rabbit Polyclonal Antibody
SKU:CAB20200
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of coronavirus NSP1 (YP_009742608.1).
Sequence:MESL VPGF NEKT HVQL SLPV LQVR DVLV RGFG DSVE EVLS EARQ HLKD GTCG LVEV EKGV LPQL EQPY VFIK RSDA RTAP HGHV MVEL VAEL EGIQ YGRS GETL GVLV PHVG EIPV AYRK VLLR KNGN KGAG GHSY GADL KSFD LGDE LGTD PYED FQEN WNTK HSSG VTRE LMRE LNGG
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Positive Sample:293T
Calculated MW:141kDa
Observed MW:20kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS-CoV-2 NSP1 Rabbit pAb (CAB20200) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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