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SARS-CoV-2 3CLpro Rabbit Polyclonal Antibody (CAB20198)

SKU:
CAB20198
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
$167 - $503
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Description

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SARS-CoV-2 3CLpro Rabbit Polyclonal Antibody (CAB20198)

The Anti-SARS-CoV-2 3CLpro Antibody (CAB20198) is a crucial tool for researchers studying the SARS-CoV-2 virus, which has caused a global pandemic. This antibody, raised in rabbits, specifically targets the 3CLpro protein of the virus and is highly reactive with human samples. It has been validated for use in various applications, including Western blotting, enabling researchers to detect and analyze the 3CLpro protein in different cell types.The 3CLpro protein, also known as the main protease, plays a vital role in viral replication and is a potential target for antiviral drug development.

By studying this protein and understanding its function, researchers can gain valuable insights into the mechanisms of SARS-CoV-2 infection and explore potential therapeutic strategies to combat the virus.Overall, the Anti-SARS-CoV-2 3CLpro Antibody (CAB20198) is an essential tool for researchers looking to advance our knowledge of the SARS-CoV-2 virus and potentially contribute to the development of effective treatments and preventive measures against COVID-19.

Product Name:SARS-CoV-2 3CLpro Rabbit Polyclonal Antibody
SKU:CAB20198
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of coronavirus 3CLpro (YP_009725295.1).
Sequence:MESL VPGF NEKT HVQL SLPV LQVR DVLV RGFG DSVE EVLS EARQ HLKD GTCG LVEV EKGV LPQL EQPY VFIK RSDA RTAP HGHV MVEL VAEL EGIQ YGRS GETL GVLV PHVG EIPV AYRK VLLR KNGN KGAG GHSY GADL KSFD LGDE LGTD PYED FQEN WNTK HSSG VTRE LMRE LNGG AYTR YVDN NFCG PDGY PLEC IKDL LARA GKAS CTLS EQLD FIDT KRGV YCCR EHEH EIAW YTER SEKS YELQ TPFE IKLA KKFD TFNG ECPN FVFP LNSI IKTI QPRV EKKK LDGF MGRI RSVY PV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:2000 - 1:6000 IF/ICC,1:50 - 1:200
Positive Sample:293T
Calculated MW:141kDa
Observed MW:30-35kDa

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody (CAB20198) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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