The Anti-SAE1 Antibody (CAB0891) is a polyclonal antibody designed for research involving the SAE1 protein, a critical component of the SUMOylation pathway. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds to the SAE1 protein, enabling detection and analysis in various cell types.The SUMOylation pathway is involved in the post-translational modification of proteins, regulating various cellular processes such as DNA repair, gene expression, and protein stability. SAE1 is a key enzyme in this pathway, essential for the activation of SUMO proteins and subsequent conjugation to target proteins.
Research into SAE1 and SUMOylation is important for understanding cellular signaling, protein interactions, and disease mechanisms.Studies on SAE1 have implications in multiple fields, including cancer research, neurodegenerative diseases, and immune regulation. By targeting SAE1, researchers can gain insights into the role of SUMOylation in disease progression and potentially identify new therapeutic targets. The Anti-SAE1 Antibody (CAB0891) is a valuable tool for investigating the function and expression of SAE1 in physiological and pathological conditions.
Product Name:
SAE1 Rabbit Monoclonal Antibody
SKU:
CAB0891
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SAE1 (Q9UBE0).
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Purification Method:
Affinity purification
Gene ID:
10055
Clone Number:
ARC1846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SAE1 Rabbit mAb (CAB0891) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
