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RPS6KA1/RPS6KA2/RPS6KA3 Rabbit Polyclonal Antibody (CAB20285)

SKU:
CAB20285
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:RPS6KA1/RPS6KA2/RPS6KA3 Rabbit Polyclonal Antibody
SKU:CAB20285
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human RPS6KA1/RPS6KA2/RPS6KA3 (NP_002944.2).
Sequence:LGIL LYTM LAGY TPFA NGPS DTPE EILT RIGS GKFT LSGG NWNT VSET AKDL VSKM LHVD PHQR LTAK QVLQ HPWV TQKD KLPQ SQLS HQDL QLVK GAMA A
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Positive Sample:Jurkat,C6,Mouse brain
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, nucleoplasm
Observed MW:83kDa

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification Method:Affinity purification
Gene ID:6195/ 6196/ 6197
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit pAb (CAB20285) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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