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RPL7A Rabbit Monoclonal Antibody (CAB19247)

SKU:
CAB19247
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
RPL7A
L7A
SURF3
TRUP
ribosomal protein L7a
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:RPL7A Rabbit Monoclonal Antibody
SKU:CAB19247
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPL7A (P62424).
Sequence:MPKG KKAK GKKV APAP AVVK KQEA KKVV NPLF EKRP KNFG IGQD IQPK RDLT RFVK WPRY IRLQ RQRA ILYK RLKV PPAI NQFT QALD RQTA TQLL KLAH KYRP ETKQ EKKQ RLLA RAEK KAAG KGDV PTKR PPVL RAGV NTVT TLVE NKKA QLVV IAHD VDPI E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:L7A; eL8; TRUP; SURF3; PLA-X polypeptide; RPL7A
Positive Sample:293T,PC-12,Hep G2,NIH/3T3,Raji,Mouse lung,Mouse liver,Rat lung,Rat liver
Conjugate:Unmodified
Cellular Localization:Cytoplasm, Cytosol, Cytosolic ribosome, Focal adhesion, Nucleolus, Nucleus
Calculated MW:30kDa
Observed MW:32kDa

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Purification Method:Affinity purification
Gene ID:6130
Clone Number:ARC2407
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using RPL7A antibody (CAB19247) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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