The RNF14 Polyclonal Antibody (CAB20913) is a valuable tool for researchers studying the regulatory role of RNF14 in cellular processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RNF14 protein, this antibody enables the detection and analysis of RNF14 in various cell types, making it ideal for investigations in molecular biology and cancer research.RNF14, also known as a RING finger protein, is known to be involved in the ubiquitination process, which plays a critical role in protein degradation and regulation.
Its function in cell signaling pathways and protein turnover makes it a key player in processes such as cell cycle regulation and DNA repair. Research into the activity of RNF14 can provide valuable insights into disease mechanisms and potential therapeutic targets, especially in the fields of cancer biology and molecular medicine.
Product Name:
RNF14 Monoclonal Antibody
SKU:
CAB20913
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human RNF14 (Q9UBS8).
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
9604
Clone Number:
ARC2884
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RNF14 antibody (CAB20913) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
