The Ring1A Polyclonal Antibody (CAB19750) is a versatile tool for researchers studying Ring1A, a key player in epigenetic regulation and gene expression. Raised in rabbits, this antibody is highly specific for human samples and is validated for use in Western blot applications. By binding to the Ring1A protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for studies in molecular biology and developmental biology.Ring1A is a member of the Polycomb group proteins, which play a crucial role in controlling gene expression through chromatin modification.
Its involvement in epigenetic regulation makes it a valuable target for research into various biological processes, including cell differentiation, stem cell biology, and cancer development. Understanding the functions of Ring1A is essential for unraveling the complexities of gene regulation and potentially identifying new therapeutic targets in diseases related to aberrant gene expression.
Product Name:
RING1A Rabbit Monoclonal Antibody
SKU:
CAB19750
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RING1A A (Q06587).
Sequence:
DPNF DALI SKIY PSRE EYEA HQDR VLIR LSRL HNQQ ALSS SIEE GLRM QAMH RAQR VRRP IPGS DQTT TMSG GEGE PGEG EGDG EDVS SDSA PDSA PGPA P
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RNF1; RING1A
Positive Sample:
HeLa,HCT116
Conjugate:
Unconjugated
Cellular Localization:
Cytosol, Nuclear speck, Nucleoplasm, Nucleus
Calculated MW:
42kDa
Observed MW:
54kDa
This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.
Purification Method:
Affinity purification
Gene ID:
6015
Clone Number:
ARC2278
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using RING1A Rabbit mAb (CAB19750) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
