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[KO Validated] RhoA Rabbit Polyclonal Antibody (CAB18695)

SKU:
CAB18695
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ARHA
ARH12
RHO12
EDFAOB
RHOH12
Research Area:
Cell Cycle
$167
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] RhoA Rabbit Polyclonal Antibody
SKU:CAB18695
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoA (NP_001655.1).
Sequence:NIPE KWTP EVKH FCPN VPII LVGN KKDL RNDE HTRR ELAK MKQE PVKP EEGR DMAN RIGA FGYM ECSA KTKD GVRE VFEM ATRA ALQA RRGK KKSG CLVL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ARHA; ARH12; RHO12; EDFAOB; RHOH12; [KD Validated] RhoA
Positive Sample:HeLa,HUVEC,PC-12,Mouse brain
Cellular Localization:Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Cytoplasmic side, Lipid-anchor, Midbody, cell cortex, cytoskeleton, lamellipodium.
Calculated MW:22kDa
Observed MW:22kDa

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.

Purification Method:Affinity purification
Gene ID:387
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using [KD Validated] RhoA Rabbit pAb (CAB18695) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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