The Anti-RhoA (KO Validated) Antibody (CAB18695) is a crucial tool for researchers studying RhoA, a small GTPase protein involved in cell signaling and regulation of the actin cytoskeleton. This antibody, produced through knockout validation, specifically targets RhoA and is highly reactive with human samples, making it ideal for Western blot applications.RhoA plays a pivotal role in various cellular processes, including cell migration, cell adhesion, and cell cycle progression. Dysregulation of RhoA has been associated with cancer metastasis, cardiovascular diseases, and neurological disorders.
Therefore, understanding the function and regulation of RhoA is essential for developing targeted therapies and interventions.By utilizing the Anti-RhoA (KO Validated) Antibody (CAB18695), researchers can accurately detect and analyze RhoA expression in different cell types, providing valuable insights into its role in physiological and pathological conditions. This antibody is a reliable tool for advancing research in cell biology, cancer biology, and drug discovery related to RhoA signaling pathways.
Product Name:
[KO Validated] RhoA Rabbit Polyclonal Antibody
SKU:
CAB18695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoA (NP_001655.1).
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
Purification Method:
Affinity purification
Gene ID:
387
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] RhoA Rabbit pAb (CAB18695) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
