The RCHY1 Polyclonal Antibody (CAB19287) is an essential tool for researchers studying the RCHY1 protein, a key player in the regulation of cellular processes such as protein degradation and tumor suppression. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it a reliable tool for Western blot applications. By targeting the RCHY1 protein, this antibody allows for accurate detection and analysis in various cell types, enabling researchers to explore its role in cancer biology and other diseases.
RCHY1, also known as RING finger and CHY zinc finger domain-containing protein 1, is involved in ubiquitination processes that target proteins for degradation, contributing to the regulation of cell growth and survival. Dysregulation of RCHY1 has been implicated in various cancers and other conditions, highlighting its potential as a therapeutic target. By unraveling the mechanisms of RCHY1 action, researchers can gain valuable insights into disease processes and potentially identify novel treatment strategies.
Product Name:
RCHY1 Rabbit Monoclonal Antibody
SKU:
CAB19287
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-261 of human RCHY1 (Q96PM5).
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
25898
Clone Number:
ARC2469
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HCT116 cells, using RCHY1 antibody (CAB19287) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
