Rap1GAP Rabbit Monoclonal Antibody (CAB3621)
- SKU:
- CAB3621
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- RAP1GA1
- RAP1GAP1
- RAP1GAPII
- RAPGAP
- Research Area:
- Cell Biology
Description
| Product Name: | Rap1GAP Rabbit Monoclonal Antibody |
| SKU: | CAB3621 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736). |
| Sequence: | MIEK MQGS RMDE QRCS FPPP LKTE EDYI PYPS VHEV LGRE GPFP LILL PQFG GYWI EGTN HEIT SIPE TEPL QSPT TKVK LECN PTAR IYRK HFLG KEHF |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
| Synonyms: | RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII; Rap1GAP |
| Positive Sample: | BxPC-3,U-87MG,Mouse brain,Rat brain |
| Conjugate: | Unconjugated |
| Cellular Localization: | Golgi apparatus membrane, Peripheral membrane protein. |
| Calculated MW: | 73kDa |
| Observed MW: | 95kDa |
This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.
| Purification Method: | Affinity purification |
| Gene ID: | 5909 |
| Clone Number: | ARC2058 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using Rap1GAP Rabbit mAb (CAB3621) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s. |
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