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RAD54L Rabbit Polyclonal Antibody (CAB20181)

SKU:
CAB20181
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
HR54
hHR54
RAD54A
hRAD54
€139 - €419
Frequently bought together:

Description

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Product Name:RAD54L Rabbit Polyclonal Antibody
SKU:CAB20181
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 648-747 of human RAD54L (Q92698).
Sequence:EEQD VERH FSLG ELKE LFIL DEAS LSDT HDRL HCRR CVNS RQIR PPPD GSDC TSDL AGWN HCTD KWGL RDEV LQAA WDAA STAI TFVF HQRS HEEQ RGLR
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:HR54; hHR54; RAD54A; hRAD54; RAD54L
Positive Sample:Mouse testis,Mouse spleen,Mouse thymus,Rat testis
Conjugate:Unconjugated
Cellular Localization:nucleoplasm, nucleus
Calculated MW:84kDa
Observed MW:84kDa

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.

Purification Method:Affinity purification
Gene ID:8438
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RAD54L Rabbit pAb (CAB20181) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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