The RAD54L Antibody (CAB20181) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in-house using rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications.RAD54L is a crucial factor in maintaining genomic stability and promoting efficient DNA repair mechanisms, making it essential for cell survival and preventing the development of genetic mutations. By targeting the RAD54L protein, researchers can investigate its role in DNA damage response pathways and explore its potential as a therapeutic target for cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
RAD54L Antibody
SKU:
CAB20181
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HR54, hHR54, RAD54A, hRAD54, RAD54L
Positive Sample:
Mouse testis, Mouse spleen, Mouse thymus, Rat testis
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
84kDa
Observed MW:
84kDa
The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
8438
RRID
AB_2862968
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RAD54L Rabbit pAb (CAB20181) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
