The RAD51D Monoclonal Antibody (CAB20869) is a valuable tool for researchers studying RAD51D, a key player in DNA repair processes. This antibody, produced in mice, is highly specific to human RAD51D and has been validated for use in multiple applications, including Western blot and immunofluorescence.RAD51D is essential for homologous recombination, a critical mechanism for DNA repair and maintenance of genomic stability. Dysregulation of RAD51D has been linked to various diseases, including cancer and genetic disorders. By targeting RAD51D, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.
The RAD51D Monoclonal Antibody provides researchers with a reliable tool for detecting and analyzing RAD51D expression in different cell types and tissues. Its high specificity and sensitivity make it ideal for a wide range of research applications in the fields of genetics, oncology, and molecular biology. By studying RAD51D, researchers can contribute to the advancement of knowledge in DNA repair pathways and potentially lead to the development of novel treatment strategies for diseases associated with DNA damage.
Product Name:
Rad51D Monoclonal Antibody
SKU:
CAB20869
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Rad51D (O75771).
293T, Hep G2, Mouse testis, Mouse kidney, Mouse liver, Rat thymus
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule organizing center, Telomere
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene.
Purification Method:
Affinity purification
Gene ID:
5892
Clone Number:
ARC2800
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various lysates, using Rad51D antibody (CAB20869) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
