The Rad21 Polyclonal Antibody (CAB19749) is a valuable tool for researchers studying the Rad21 protein, a key player in the regulation of cell division and DNA repair processes. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the Rad21 protein, this antibody enables detection and analysis in various cell types, making it an essential reagent for studies in genetics, cell biology, and cancer research.
Rad21, a component of the cohesin complex, is essential for the faithful segregation of chromosomes during cell division. Its role in maintaining genome stability and ensuring proper DNA repair processes makes it a crucial target for investigation in various disease contexts, including cancer and developmental disorders. By understanding the function of Rad21, researchers can gain insights into the mechanisms underlying these diseases and potentially develop targeted therapies to treat them.
Product Name:
Rad21 Rabbit Monoclonal Antibody
SKU:
CAB19749
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 532-631 of human Rad21 (O60216).
Condensed nuclear chromosome, Cytosol, nuclear matrix, Nucleoplasm, Nucleus, Spindle pole
Calculated MW:
72kDa
Observed MW:
130kDa
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Purification Method:
Affinity purification
Gene ID:
5885
Clone Number:
ARC2276
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Chromatin immunoprecipitation was performed with 10.7 μg of cross-linked chromatin from A-549 cells using 5 μg of Rad21 Rabbit mAb (CAB19749). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of Rad21 across chromosome 8 (upper panel) and the genomic region encompassing MYC, a representative gene enriched in Rad21 (lower panel).
