The Anti-Rab11A/Rab11B Antibody (CAB20286) is a valuable tool for researchers studying Rab11A and Rab11B proteins, which are involved in intracellular membrane trafficking and recycling processes. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Rab11A and Rab11B proteins, researchers can accurately detect and analyze these proteins in various cell types.Rab11A and Rab11B are essential for maintaining cell polarity and regulating endocytic pathways, making them important targets for understanding cellular processes such as membrane trafficking, protein recycling, and organelle dynamics.
Research into Rab11A and Rab11B has implications in various fields, including cell biology, molecular biology, and cancer research.By utilizing the Anti-Rab11A/Rab11B Antibody (CAB20286), researchers can gain valuable insights into the functions and mechanisms of Rab11A and Rab11B proteins, ultimately advancing our understanding of intracellular membrane trafficking and its role in cellular physiology and disease.
Product Name:
RAB11A/RAB11B Rabbit Polyclonal Antibody
SKU:
CAB20286
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/RAB11B (NP_004654.1).
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
Purification Method:
Affinity purification
Gene ID:
8766/ 9230
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RAB11A/RAB11B Rabbit pAb (CAB20286) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
