The Anti-Pyrophosphatase-1 Polyclonal Antibody (CAB20942) is a valuable tool for researchers studying pyrophosphatase-1, an enzyme involved in hydrolysis of inorganic pyrophosphate. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to pyrophosphatase-1, this antibody enables accurate detection and analysis of the enzyme in various cell types, making it ideal for investigations in biochemistry and molecular biology.Pyrophosphatase-1 plays a crucial role in cellular metabolism by catalyzing the hydrolysis of inorganic pyrophosphate, a byproduct of many biochemical reactions.
Understanding the function and regulation of this enzyme is essential for unraveling its impact on processes like nucleic acid synthesis, energy metabolism, and phosphate homeostasis. Researchers studying diseases related to pyrophosphatase-1 dysregulation, such as pyrophosphate arthropathy and certain metabolic disorders, can benefit from the use of this antibody in their experimental studies.Overall, the Anti-Pyrophosphatase-1 Polyclonal Antibody (CAB20942) offers a reliable tool for investigating the physiological roles and pathological implications of pyrophosphatase-1, paving the way for advancements in drug discovery and therapeutic interventions targeting this enzyme.
Product Name:
Pyrophosphatase 1 Monoclonal Antibody
SKU:
CAB20942
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pyrophosphatase 1 (Q15181).
MCF7,OVCAR3,Mouse large intestine,Mouse liver,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Purification Method:
Affinity purification
Gene ID:
5464
Clone Number:
ARC2928
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Pyrophosphatase 1 antibody (CAB20942) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
