The Anti-Pseudouridine 5'-Ribosyluracil Antibody (CAB20988) is a polyclonal antibody specifically designed for research involving the detection and analysis of pseudouridine 5'-ribosyluracil, an important post-transcriptional modification of RNA. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.Pseudouridine 5'-ribosyluracil is a modified nucleoside found in various types of RNA and plays a crucial role in regulating RNA stability, structure, and function. This antibody binds specifically to pseudouridine 5'-ribosyluracil, enabling researchers to study and analyze this modification in different cell types and tissues.
It is an essential tool for investigations into RNA biology, gene expression regulation, and RNA modification pathways.The Anti-Pseudouridine 5'-Ribosyluracil Antibody is ideal for studies in molecular biology, RNA research, and epigenetics, providing valuable insights into the role of RNA modifications in cellular processes and disease development. By targeting pseudouridine 5'-ribosyluracil, researchers can further our understanding of RNA biology and potentially uncover new therapeutic targets for diseases associated with RNA dysregulation.
Product Name:
Pseudouridine/5-ribosyluracil Monoclonal Antibody
SKU:
CAB20988
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to Pseudouridine / 5-ribosyluracil / Y.
Tested Applications:
ELISADB
Recommended Dilution:
DB,1:500 - 1:1000
Positive Sample:
Pseudouridine / 5-ribosyluracil
Observed MW:
Refer to figures
Pseudouridine (Ψ) was among the first post-transcriptional modifications discovered and is overall one of the most abundant（1）. It is present in a wide range of cellular RNAs and is highly conserved across species. Ψ is derived from uridine (U) via base-specific isomerization catalyzed by Ψ synthases. The site-specific pseudouridylation goes through either snoRNA-dependent (requires H/ACA RNP) or -independent mechanism (requires pseudouridine synthase (PUS) family enzymes)（2）. It has an extra hydrogen-bond donor at its non-Watson-Crick edge. When incorporated into RNA, Ψ can alter RNA secondary structure by increasing base stacking, improving base pairing and rigidifying sugar-phosphate backbone5. The chemical and physical properties of RNA can be altered with the incorporation of Ψ, which could contribute to subsequent cellular functions.
Purification Method:
Affinity purification
Gene ID:
CAS: 1445-07-4
Clone Number:
ARC50719
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Dot-blot analysis of different sorts of chemical compounds using Pseudouridine / 5-ribosyluracil Rabbit mAb (CAB20988) at 1:1000 dilution.
