The Anti-Pseudouridine-5'-Ribosyluracil Antibody (CAB18872) is a valuable tool for researchers studying RNA modification. This antibody, generated in rabbits, specifically targets pseudouridine-5'-ribosyluracil (Ψ) in RNA molecules. Pseudouridine is a commonly occurring modified nucleoside in RNA, and the antibody is highly reactive with samples from various species, including human, mouse, and rat.With its validated application in techniques such as immunofluorescence and immunoprecipitation, the Anti-Pseudouridine-5'-Ribosyluracil Antibody enables detection and quantification of Ψ-modified RNA in cells and tissues.
This makes it an essential tool for studying the role of RNA modifications in gene expression, RNA processing, and disease development.Researchers interested in unraveling the functions of pseudouridine in various biological processes, including mRNA translation, stability, and splicing, will find this antibody instrumental in their investigations. Its specificity for Ψ-modified RNA makes it a valuable asset for studies in molecular biology, epigenetics, and RNA biology.
Product Name:
Pseudouridine Rabbit Polyclonal Antibody
SKU:
CAB18872
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Chemical compounds corresponding to Pseudouridine / 5-ribosyluracil / Y.
Tested Applications:
ELISADB
Recommended Dilution:
DB,1:3000 - 1:10000
Observed MW:
Refer to figures
Pseudouridine is the most abundant post-transcriptional RNA modification, which presents mostly in non-coding RNAs such as tRNA, rRNA, snRNA and snoRNA. Pseudouridine impacts various aspects of RNA biology, conferring distinct structural and functional properties to the RNA molecules that it decorates.? It is also reported that pseudouridine has been found in mRNA in both yeast and human by sequencing analysis. Replacing uridine to pseudouridine enhances structural stability of RNA, and is expected to affect rRNA processing, translation and pre-mRNA splicing. Also, aberrant pseudouridylation contributes to a variety of human diseases, including cancer and genetic disorders. Dysregulation of the pseudouridine epitranscriptome can arise from mutations and abnormal expression of pseudouridylation machinery, impacting protein translation and other cellular processes.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Dot-blot analysis of all sorts of chemical compounds using Pseudouridine / 5-ribosyluracil antibody (CAB18872) at 1:5000/1:10000 dilution
