Proteinase K Rabbit Polyclonal Antibody (CAB20453)
The Proteinase K Polyclonal Antibody (CAB20453) is a valuable tool for researchers studying Proteinase K, a serine protease commonly used in molecular biology applications. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot assays. By binding specifically to Proteinase K, this antibody enables precise detection and analysis of the enzyme in a variety of cell types, making it an ideal choice for studies focused on enzyme activity and protein degradation mechanisms.Proteinase K is widely utilized in molecular biology research for its ability to efficiently digest proteins, making it a crucial component in various experimental techniques such as DNA and RNA extraction.
Understanding the function and regulation of Proteinase K is essential for optimizing experimental protocols and ensuring accurate results in biological research. This antibody provides researchers with a reliable tool for investigating the role of Proteinase K in biological processes and developing innovative approaches in molecular biology and biochemistry.
Product Name:
Proteinase K Rabbit Polyclonal Antibody
SKU:
CAB20453
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human Proteinase K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
Proteinase K
Conjugate:
Unconjugated
Observed MW:
60kDa
Proteinase K is an endopeptidase belonging to the subtilisin group of serine proteases. Proteinase K was isolated from the mold (Parengyodontium album or previously known as Tritirachium album). Proteinase K has five cysteines. Four of the cysteine residues form two disulfide bonds (34-124 and 179-248, respectively) and one lies below one of the catalytic triads. Proteinase K often binds two calcium ions required for full enzymatic activity. In molecular biology laboratories, proteinase K is useful during preparation of DNA or RNA samples by degrading and inactivating proteins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Proteinase K, using Proteinase K Rabbit pAb (CAB20453) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: .
