null

PRDM9 Rabbit Polyclonal Antibody (CAB20340)

SKU:
CAB20340
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PFM6
KMT8B
MSBP3
ZNF899
MEISETZ
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PRDM9 Rabbit Polyclonal Antibody
SKU:CAB20340
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 413-525 of human PRDM9 (NP_064612.2).
Sequence:SQNF PGPS ARKL LQPE NPCP GDQN QEQQ YPDP HSRN DKTK GQEI KERS KLLN KRTW QREI SRAF SSPP KGQM GSCR VGKR IMEE ESRT GQKV NPGN TGKL FVGV GISR IAKV K
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PFM6; KMT8B; MSBP3; ZNF899; MEISETZ; PRDM9
Positive Sample:Mouse stomach,Rat testis
Conjugate:Unconjugated
Cellular Localization:nucleoplasm, nucleus
Calculated MW:103kDa
Observed MW:103kDa

The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described.

Purification Method:Affinity purification
Gene ID:56979
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using PRDM9 antibody (CAB20340) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Related Products