The PLA2G2A Polyclonal Antibody (CAB19252) is an essential tool for studying the PLA2G2A protein, a member of the phospholipase A2 enzyme family that plays a key role in inflammatory responses and lipid metabolism. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot experiments. By binding to the PLA2G2A protein, this antibody allows for precise detection and analysis in various cell types, making it an ideal choice for immunology and cancer research studies.PLA2G2A is a crucial enzyme involved in the production of lipid mediators that regulate inflammation and immune responses.
Dysregulation of PLA2G2A activity has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. Therefore, investigating the function and regulation of PLA2G2A is essential for developing new therapeutic strategies targeting these health challenges. The PLA2G2A Polyclonal Antibody provides researchers with a valuable tool to advance our understanding of the role of PLA2G2A in health and disease.
Product Name:
PLA2G2A Rabbit Monoclonal Antibody
SKU:
CAB19252
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 65-144 of human PLA2G2A (P14555).
HT-29,HepG2,DU145,Mouse liver,Mouse spleen,Mouse small intestine,Rat spleen,Rat small intestine,Rat liver
Conjugate:
Unmodified
Cellular Localization:
Membrane, Peripheral membrane protein.
Calculated MW:
16kDa
Observed MW:
15kDa
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5320
Clone Number:
ARC2416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLA2G2A antibody (CAB19252) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
