The Anti-PKMYT1 Antibody (CAB20525) is a valuable tool for researchers studying PKMYT1, a critical protein involved in cell cycle regulation and checkpoint control. This antibody, produced in rabbits, exhibits high specificity to human samples and is validated for use in Western blot applications. By binding to the PKMYT1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential reagent for studies in cell biology and cancer research.PKMYT1, also known as Wee1B kinase, plays a pivotal role in cell cycle progression by phosphorylating and inhibiting CDK1, thereby preventing premature entry into mitosis.
Dysregulation of PKMYT1 activity is often observed in cancer, making it a promising target for therapeutic interventions aimed at inducing cell cycle arrest and apoptosis in tumor cells. By investigating the functions of PKMYT1, researchers can gain valuable insights into the molecular mechanisms underlying cancer development and potential treatment strategies.
Product Name:
PKMYT1 Rabbit Polyclonal Antibody
SKU:
CAB20525
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 75-362 of human PKMYT1 (NP_004194.3).
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
9088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PKMYT1 antibody (CAB20525) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
